Brentford have rejected an offer from Middlesbrough for Jota.
Boro offered £4.5m plus add-ons for the Spanish playmaker, who has attracted interest from several clubs.
The 25-year-old impressed after returning to Brentford in January from a loan spell at Eibar.
Jota scored 12 goals during the second half of last season and is under contract at Griffin Park until next summer.
He has featured in three of Brentford’s four Championship matches this season.