Chris Mepham has withdrawn from the Wales squad for the Nations League match against Denmark on Friday.
Sheffield United’s Kieron Freeman has been called up to replace him for the Denmark game at Cardiff and next Tuesday’s friendly with Albania in Elbasan.
Brentford defender Mepham pulled out of last month’s high-profile friendly against Spain and the Nations League tie with the Republic of Ireland after picking up a knock in training.
Mepham has played four times for the Bees since the last international break, but he has suffered a recurrence of the unspecified injury that ruled him out last month.
Mepham, 20, has impressed since making his international debut at the China Cup in March.
The Hammersmith-born centre-back started both Nations League games against the Republic and Denmark in September.