Thomas Frank admitted Brentford were second best in their defeat by Barnsley.
And the Bees boss has demanded an instant response in Wednesday’s derby against QPR.
“I have just said to the players that we lost to a better team today and it is all about how we react to that and we need to bounce back against QPR,” he said.
“Barnsley deserve a lot of praise. We have been so consistent for 21 games, but at the end of the day we lost it fair and square.
!There were a decisive few moments – if Josh Dasilva and Saman Ghoddos had scored their shots in the first half then it may have been different. And then we made a mistake for them to go 2-0 up, they scored and it’s a different game.
“There was no lack of effort from the players, they put everything out there and there was a big determination from all of them. We are in such a good place, but we lost to a team who made it difficult when they hit their high level.”
The defeat ended Brentford’s record-breaking unbeaten 21-game Championship run and Frank said: “I am frustrated we lost but not frustrated overall.
“For me it was inevitable it would happen at some stage – we knew that. We just hoped that today wouldn’t be the day.
“We had hoped to go one more game, one more game, one more game. But we know in this league you can lose to every team in this division, even if you are at the highest level.”
It was the fourth game in a row in which Brentford fell behind early on.
Frank said: “I knew that because three times we had come back from a goal down that we would be able to do it for a fourth time.
“I believed in that, but at 2-0 I thought it was going to be very difficult.”
He added: “Whenever we play QPR it is a big game – whether it is a relegation battle, a middle of the table game or when one team is high in the table.
“It means the world to both clubs’ fans and what an opportunity for us to bounce back against them.
“The players know every single time they go out they are playing for the fans and for the club but also for the team, who want to do well and try to achieve the best they can.
“I am in no doubt that they will come flying out on Wednesday night.”